Glycoconjugates distribution in the lateral walls of spinal cord during mouse morphogenesis using lectin histochemical method

Background and Objective: Different organizers are involved in spinal cord development and differentiation by sending various messages. Specific glycoconjugates secreted from the cells of lateral wall of spinal cord can also act as neurogenesis and neural differentiation messengers. This study was carried out to determine the distribution of sugar compounds in the lateral walls of spinal cord during mice morphogenesis using lectin histochemistry method

Methods: In this experimental study, sections of BALB/c mice from 10-16 embryonic days were fixed in formalin and then histological sections were prepared. Tissue samples for reaction to the glycoconjugates were incubated with DBA, OFA, GSA1B4 and MPA lectins. Alcian blue with pH equal 2.5 was used for background staining

Results: DBA lectin did not react with the lateral wall of the spinal cord. MPA lectin showed severe reaction but consistent, especially in nerve fibers of the lateral wall of spinal cord. GSA1B4 lectin showed weak reaction in the cells and nerve fibers of the spinal cord, but severe reaction was clearly observed in blood vessels. OFA lectin showed severe reaction with -L-Fucose terminal sugar in the lateral walls of the spinal cord in early stages of morphogenesis

Conclusion: The most reaction in the lateral walls of the spinal cord was related to OFA, which reflects the importance of fucose terminal sugar by connecting [16] to the penultimate sugar N-acetyl-D-glocosamin [Glc-Nac] in the development of spinal cord. Due to severe reaction of GSA1B4 to blood vessels of spinal cord, use of this lectin for vascular studies, is recommended

[ anatomical variation of extensor indicis muscle: a case report]

The extensor indicis is a narrow, elongated skeletal muscle in the deep layer of the dorsal forearm that originates from the one third of the distal posterior surface of the shaft of ulna below the origin of the extensor pollicis longos and interosseous membrane. It runs through the fourth tendon compartment with the extensor digitorum under the extensor retinaculum. The extensor indicis joins to the ulnar side of the extensor digitorum communis opposite the head of the second metacarpal bone. This muscle allows independent extension of the index finger. In a cadaver dissection we observed two extensor indicis muscles with the same origin but two separate belleis and tendons. One of them was attached to the dorsum of the index finger and another one to the tendon of extensor digitorum muscle and to the index finger via extensor expansion

[Distribution of glycoconjugates terminal sugars during neurohypophysis development in rat]

The neurohypophysis originates from the floor of diencephalon. Its development controles by several cellular interactions that mediated by some molecules such as cell surface and extra cellular matrix Glycoconjugates terminal sugars. In this study we used lectin histochemichal technique to evaluate distribution of the Glycoconjugates and their changes during development of neurohypophysis. This experimental study carried on 40 female and 20 male adult Rats. After mating and appointment day zero of pregnancy, pregnant Rats were sacrificed from days 10-20 of gestation and their embryos were collected for histochemical study. The serial section of head specimens were fixed and incubated with different HRP-lectins from Orange fungus [OFA] Vicica villosa [VVA], Glycine max [SBA], Wistaria floribunda [WFA], peanut [PNA], Griffonia simplicfolia [GSA1-B4], Lotus tetragonolobus [LTA] and Ulex Europeus [UEA-1]. OFA, LTA and UEA-1 lectins are specific for terminal sugars alpha -L-Fucose and WFA, SBA, VVA and PNA are specific for D-GalNAc, alpha, beta -D-GalNAc and GalNAc, D-Gal-[beta -1-3]- DGalNAc of complex glycoconjugates respectively. Our findings demonstrated that the reaction of neurohypophysis cells with OFA initiated from gestational GD10 and increased to GD15 [P<0.05] and then increased to GD17 [P<0.05]. A few cells of neurohypophysis reacted with PNA from GD13 to GD16 and decreased afterward [P<0.05]. Some cells of neurohypophysis reacted with SBA from GD14 to GD18 and decreased afterward [P<0.05]. Reacting of many cells of neurohypophysis with WFA started on GD13 and increased to GD15 [P<0.05] and then decreased afterward [P<0.05]. Neurohypophysis cells showed no reaction with the UEA-1, LTA, VVA and GSA1-B4 lectins. The expression of Glycoconjugates with terminal sugars alpha -L-Fucose, alpha, beta -D-GalNAc and D- Gal- [beta -1-3]- D-GalNAc have importanct role and special spatiotemporal situation in neurohypophysis development